Biohydrogen and Methane Production from Sugarcane Leaves Pretreated by Deep Eutectic Solvents and Enzymatic Hydrolysis by Cellulolytic Consortia

This study determined the optimal conditions for deep eutectic solvent (DES) pretreatment of sugarcane leaves and best fermentation mode hydrogen methane production from DES-pretreated leaves. Choline chloride (ChCl):monoethanolamine (MEA) is most effective removing lignin The optimum were a ChCl: MEA molar ratio 1:6, 120 °C, 3 h, substrate-to-DES solution 1:12. Under these conditions, 86.37 ± 0.36% removal 73.98 0.42% hemicellulose achieved, whereas 84.13 0.77% cellulose was recovered. At substrate loading 4 g volatile solids (VS), simultaneous saccharification (SSF) separate hydrolysis (SHF) processes yielded maximum productions 3187 202 2135 315 mL H2/L, respectively. In second stage, produced using hydrogenic effluent. SSF 5923 251 CH4/L, SHF 3583 128 CH4/L. one-stage process, 4067 320 CH4/L with VS achieved process. proved to be more efficient than producing in two-stage process as well